Synchrotron micro-CT imaging of stemcell – University of Copenhagen

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Synchrotron μ-CT Imaging of stemcell- or substitute mediated bone, blood vessels and implant integration

A) Synchrotron μ-CT imaging of substitute mediated bone formation in dental implant integration

The part aims at visualizing and describing bone microarchitecture in close proximity to a dental titanium implant surface, and evaluate bone density in a radial distance from an implant surface, after immediate implant installation and bone substitute augmentation.

B) Synchrotron μ-CT imaging of stem cell mediated bone and blood vessels

Development of an in vivo model comprised of fat-derived stroma cells isolated from mice, differentiated into mature osteoblasts ex vivo and subsequently transplantd back into the live mice with and without titanium granules (oxidized and non-oxidized) as osteogenic stimulus. Specimens will be examined with non-decalcified histology/histomorphometry, X-ray microscopy and SRμCT. Furthermore, a collagen culture/scaffold model in which bone cells can be cultured and differentiated from fat-deried stroma cells into mature osteoblasts will be developed. This is complemented with examinations of the in vivo bone regenerating ability of inserting 3D cell-containing collagen matrices into critical size defects in a rat model of bone regneration.

The fundings from the CoNeXT program are dedicated to 2 part-time PhD salaries at ICM and for salary for evaluations at the Niels Bohr Institute of the scans of the different specimens from the projects A) and B)

Participants
Associate Professor, MD, dr.med. Niklas Rye Jørgensen, Institute of Clinical Medicine and head, Department of Diagnostis, Glostrup University Hospital
Professor Else M. Pinholt, DDS, dr.odont, former at the Institute of Odontology, University of Copenhagen
Professor Robert Feidenhansl', the Niels Bohr Institute, University of Copenhagen
PhD student Camilla Neldam, DDS, Institute of Clinical Medicine, University of Copenhagen
PhD student Morten Dahl, DDS, Institute of Clinical Medicine, University of Copenhagen

Collaborators
Associate Professor, MD, dr.med. Ellen Hauge, Department of Rheumatology, Aarhus University Hospital